Cullin 2 anticorps (AA 389-745)

N° du produit ABIN6719399

L’anticorps Lapin Polyclonal anti-Cullin 2 a été validé pour WB, ELISA et FACS. Il convient pour détecter Cullin 2 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Cullin 2 anticorps (AA 389-745) (ABIN6719399)

Antigène: Cullin 2 (CUL2)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cullin 2 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-Cullin 2 anticorps

Épitope: AA 389-745

Fonction: Anti-CUL2 Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-CUL2 Antibody (ABIN6719399). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human CUL2 recombinant protein (Position: E389-A745).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL

Commentaires:

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Cullin 2

Antigène: Cullin 2 (CUL2)

Autre désignation: CUL2

Sujet:

Synonyms: Cullin-2, CUL-2, CUL2

Tissue Specificity: Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Highly expressed in several malignant tissues: highly expressed in clinical melanomas, with low expression in normal skin. In melanoma, marks malignant melanoma-initiating cells (MMIC), in which clinical virulence resides as a consequence of unlimited self-renewal capacity, resulting in inexorable tumor progression and metastasis. Also highly expressed in a number of leukemia cells. Expressed in basal limbal epithelium.

Background: Cullin 2 (CUL2) is a protein that in humans is encoded by the CUL2 gene. Using immunofluorescence, it is showed that CUL2 is a cytosolic protein that can be translocated to the nucleus by VHL. By fluorescence in situ hybridization, this gene is mapped to 10p11.2-p11.1, a region reported to show loss of heterozygosity (LOH) in several forms of human cancer, including non-clear cell renal cell carcinoma. CUL2 may serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.

Poids moléculaire: 87 kDa

ID gène: 8453

UniProt: Q13617

Pathways: M Phase, Asymmetric Protein Localization, SARS-CoV-2 Protein Interactome