AIF anticorps (AA 211-260)

N° du produit ABIN6736699

Cet anticorps anti-AIF Polyclonal Lapin (ABIN6736699) détecte spécifiquement AIF dans WB, IHC et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Rat, Souris, Porc, Boeuf (Vache), Chien, Cheval, Poisson zèbre (Danio rerio) et Hamster.

Aperçu rapide pour AIF anticorps (AA 211-260) (ABIN6736699)

Antigène: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivité: Humain, Rat, Souris, Porc, Boeuf (Vache), Chien, Cheval, Poisson zèbre (Danio rerio), Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AIF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-AIF anticorps

Épitope: AA 211-260

Specificité: Human AIFM1 / AIF

Homologie: Percent identity by BLAST analysis:

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa211-260 of human AIFM1 (O95831-2, NP_665812). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Cat, Bovine, Horse, Pig (100%), Monkey, Rabbit, Guinea pig (92%), Opossum, Turkey, Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur AIF

Antigène: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Autre désignation: AIFM1 / AIF / PDCD8

Sujet: Name/Gene ID: AIFM1
Family: Apoptosis

Synonyms: AIFM1, AIF, COXPD6, PDCD8, Programmed cell death 8

ID gène: 9131

NCBI Accession: NP_665812

UniProt: O95831

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, L'effet Warburg