NR0B1 anticorps (AA 359-408)

N° du produit ABIN462167

Détail du produit anti-NR0B1 anticorps

Antigène: NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))

Épitope: AA 359-408

Reactivité: Humain, Souris, Rat, Chien, Cheval, Boeuf (Vache), Cobaye, Lapin, Singe, Poisson zèbre (Danio rerio), Poulet, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NR0B1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: Human NR0B1 / DAX-1

Réactivité croisée: Poulet, Chien, Souris, Porc, Rat (Rattus)

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa359-408 of human NR0B1 (Q9BG96, NP_000466). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Platypus, Lizard (100%), Galago, Hamster, Xenopus, Seabass, Salmon, Stickleback, Medaka (92%), Bat (91%), Zebrafish (85%), Catfish (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 2 % sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur NR0B1

Antigène: NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))

Autre désignation: NR0B1 / DAX1 (NR0B1 Produits)

Synonymes: anticorps DAX, anticorps NR0B1, anticorps dax1, anticorps AHC, anticorps AHCH, anticorps AHX, anticorps DAX-1, anticorps DAX1, anticorps DSS, anticorps GTD, anticorps HHG, anticorps NROB1, anticorps SRXY2, anticorps Ahc, anticorps Ahch, anticorps Dax1, anticorps nuclear receptor subfamily 0 group B member 1, anticorps nuclear receptor subfamily 0, group B, member 1, anticorps nuclear receptor subfamily 0 group B member 1 L homeolog, anticorps NR0B1, anticorps nr0b1, anticorps nr0b1.L, anticorps Nr0b1

Sujet: Name/Gene ID: NR0B1
Subfamily: NR0 Knirps-like
Family: NHR

Synonyms: NR0B1, AHX, AHC, AHCH, DAX1, DSS, DAX 1, DAX-1, Adrenal hypoplasia congenita, Dosage-sensitive sex reversal, GTD, HHG, Nuclear hormone receptor, NROB1, Nuclear receptor DAX-1, SRXY2, Nuclear receptor DAX1, Dax-1 receptor

ID gène: 190

NCBI Accession: NP_000466

UniProt: P51843

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling