NR1H3 anticorps (C-Term)

N° du produit ABIN462212

Détail du produit anti-NR1H3 anticorps

Antigène: NR1H3 (Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Xenopus laevis, Chien, Chévre, Cheval, Lapin, Porc, Cobaye, Singe, Poisson zèbre (Danio rerio), Roussette (Chauve-souris), Poulet, Hamster

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NR1H3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human NR1H3 / LXR Alpha

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Goat, Pig, Guinea pig, Chicken, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from C-Terminus of human NR1H3 (Q13133, NP_005684). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Trout, Salmon, Stickleback, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur NR1H3

Antigène: NR1H3 (Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3))

Autre désignation: NR1H3 / LXR Alpha (NR1H3 Produits)

Synonymes: anticorps NR1H3, anticorps LXR-a, anticorps LXRA, anticorps RLD-1, anticorps AU018371, anticorps LXR, anticorps RLD1, anticorps Unr1, anticorps LXRalpha, anticorps LXR-alpha, anticorps im:7141239, anticorps wu:fd59b01, anticorps zgc:113339, anticorps zgc:158542, anticorps nuclear receptor subfamily 1 group H member 3, anticorps nuclear receptor subfamily 1, group H, member 3, anticorps NR1H3, anticorps nr1h3, anticorps Nr1h3

Sujet: Name/Gene ID: NR1H3
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: NR1H3, Liver X receptor alpha, LXRA, LXR-a, Oxysterols receptor LXR-alpha, LXR alpha, Lxralpha, RLD-1

ID gène: 10062

NCBI Accession: NP_005684

UniProt: Q13133

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Nuclear Hormone Receptor Binding, Cellular Response to Molecule of Bacterial Origin, Hepatitis C