PLEKHH2 anticorps (AA 1043-1092)

N° du produit ABIN6741614

Détail du produit anti-PLEKHH2 anticorps

Antigène: PLEKHH2 (Pleckstrin Homology Domain Containing, Family H (With MyTH4 Domain) Member 2 (PLEKHH2))

Épitope: AA 1043-1092

Reactivité: Humain, Souris, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin, Roussette (Chauve-souris), Singe, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PLEKHH2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human PLEKHH2

Homologie: Percent identity by BLAST analysis: Dog, Bovine, Rabbit, Horse, Guinea pig (100%) Rat (92%) Chicken (86%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa1043-1092 of human PLEKHH2 (Q8IVE3, NP_742066). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Zebra finch (100%), Rat, Opossum (92%), Turkey, Chicken (86%), Xenopus, Drosophila (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur PLEKHH2

Antigène: PLEKHH2 (Pleckstrin Homology Domain Containing, Family H (With MyTH4 Domain) Member 2 (PLEKHH2))

Autre désignation: PLEKHH2 (PLEKHH2 Produits)

Synonymes: anticorps RGD1304935, anticorps si:ch73-105b23.3, anticorps PLEKHH1L, anticorps AI256725, anticorps E030001K05, anticorps mKIAA2028, anticorps pleckstrin homology, MyTH4 and FERM domain containing H2, anticorps pleckstrin homology domain containing, family H (with MyTH4 domain) member 2, anticorps Plekhh2, anticorps plekhh2, anticorps PLEKHH2

Sujet: Name/Gene ID: PLEKHH2

Synonyms: PLEKHH2, KIAA2028, PLEKHH1L

ID gène: 130271

NCBI Accession: NP_742066

UniProt: Q8IVE3