LDHB anticorps (C-Term)

N° du produit ABIN6744435

L’anticorps Lapin Polyclonal anti-LDHB a été validé pour WB, IHC et IHC (p). Il convient pour détecter LDHB dans des échantillons de Humain, Singe, Chien, Lapin et Poulet.

Aperçu rapide pour LDHB anticorps (C-Term) (ABIN6744435)

Antigène: LDHB (Lactate Dehydrogenase B (LDHB))

Reactivité: Humain, Singe, Chien, Lapin, Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LDHB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-LDHB anticorps

Épitope: C-Term

Marque: IHC-plus™

Specificité: Human LDHB

Homologie: Percent identity by BLAST analysis: Human, Dog, Rabbit, Chicken (100%) Mouse, Rat, Bovine, Horse, Pig (92%) Zebrafish (90%).

Purification: Affinity purified

Immunogène: Synthetic peptide from C-Terminus of human LDHB (P07195, NP_002291). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Ferret, Dolphin, Elephant, Panda, Dog, Rabbit, Opossum, Turkey, Chicken (100%), Galago, Mouse, Rat, Hamster, Bovine, Horse, Pig, Guinea pig (92%), Catfish (91%), Zebrafish (90%), Zebra finch (85%), Salmon (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur LDHB

Antigène: LDHB (Lactate Dehydrogenase B (LDHB))

Autre désignation: LDHB / LDH-B

Sujet: Name/Gene ID: LDHB

Synonyms: LDHB, LDH heart subunit, Lactate dehydrogenase H chain, Lactate dehydrogenase B, LDH-B, TRG-5, LDH-H, LDHBD

ID gène: 3945

NCBI Accession: NP_002291

UniProt: P07195

Pathways: L'effet Warburg