MYL6 anticorps (AA 72-121)

N° du produit ABIN6744460

Détail du produit anti-MYL6 anticorps

Antigène: MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))

Épitope: AA 72-121

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Mouton, Chien, Cobaye, Cheval, Lapin, Porc, Poisson zèbre (Danio rerio), Roussette (Chauve-souris), Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MYL6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marque: IHC-plus™

Specificité: Human MYL6

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide located between aa72-121 of human MYL6 (P60660, NP_524147). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Lizard (100%), Gibbon, Panda, Opossum (91%), Platypus (90%), Hamster, Turkey, Zebra finch, Chicken, Zebrafish (85%), Xenopus (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Détails sur MYL6

Antigène: MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))

Autre désignation: MYL6 (MYL6 Produits)

Synonymes: anticorps ESMLC, anticorps LC17, anticorps LC17-GI, anticorps LC17-NM, anticorps LC17A, anticorps LC17B, anticorps MLC-3, anticorps MLC1SM, anticorps MLC3NM, anticorps MLC3SM, anticorps zgc:103624, anticorps MLC3nm, anticorps Myln, anticorps myosin light chain 6, anticorps myosin, light chain 6, alkali, smooth muscle and non-muscle, anticorps myosin light chain 6 L homeolog, anticorps myosin, light polypeptide 6, alkali, smooth muscle and non-muscle, anticorps MYL6, anticorps Myl6, anticorps myl6, anticorps myl6.L

Sujet: Name/Gene ID: MYL6

Synonyms: MYL6, 17 kDa myosin light chain, ESMLC, LC17, LC17B, LC17-GI, LC17A, MLC3NM, MLC1SM, MLC3SM, Myosin light chain A3, Myosin light chain 3, LC17-NM, MLC-3, Myosin light polypeptide 6, Myosin light chain alkali 3

ID gène: 4637

NCBI Accession: NP_524147

UniProt: P60660