BAX anticorps (AA 3-16)

N° du produit ABIN6936465

L’anticorps anti-BAX Monoclonal Souris est utilisé pour la détection de BAX dans des échantillons de Humain et Singe. Il a été validé pour WB, IHC, IF et FACS.

Aperçu rapide pour BAX anticorps (AA 3-16) (ABIN6936465)

Antigène: BAX (BCL2-Associated X Protein (BAX))

Reactivité: Humain, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BAX est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 2D2

Détail du produit anti-BAX anticorps

Épitope: AA 3-16

Fonction: Bax (Apoptosis Marker) Antibody

Specificité: Recognizes a protein of 21 kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Aucune reactivité croisée: Souris, Rat (Rattus)

Réactivité croisée (Details): Human. Monkey. Does not react with Mouse or Rat.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur BAX

Antigène: BAX (BCL2-Associated X Protein (BAX))

Autre désignation: BAX

Sujet:

Apoptosis regulator BAX cytoplasmic isoform beta, Apoptosis regulator BAX membrane isoform alpha, Bax isoform psi, Bax protein cytoplasmic isoform delta, Bax protein cytoplasmic isoform gamma, Bax zeta, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, BCL2L4

A-549 cells, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma., Jurkat, K562

Poids moléculaire: 21kDa

ID gène: 581, 624291

UniProt: Q07812

Pathways: Signalisation p53, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response