Recombinant CTNNB1 anticorps

N° du produit ABIN6937051

Cet anticorps anti-CTNNB1 Monoclonal Lapin (Clone CTNNB1-2030R) (ABIN6937051) détecte spécifiquement CTNNB1 dans WB, IHC, IF et FACS. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Lapin, Cobaye et Hamster.

Aperçu rapide pour Recombinant CTNNB1 anticorps (ABIN6937051)

Antigène: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat, Lapin, Cobaye, Hamster

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CTNNB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: CTNNB1-2030R

Détail du produit anti-CTNNB1 anticorps

Fonction: Recombinant Catenin, beta (CTNNB1) Antibody

Specificité: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab purified by Protein A Column.

Immunogène: Recombinant full-length human β-catenin protein

Isotype: IgG, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF-7 cells. Breast carcinoma

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CTNNB1

Antigène: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Autre désignation: CTNNB1

Sujet:

Cadherin associated protein, beta 1 88 kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1

Brain, HeLa or MCF-7 cells. Breast carcinoma, Liver

Poids moléculaire: 92kDa

ID gène: 1499, 476018

UniProt: P35222

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2