Recombinant Insulin Receptor anticorps (Extracellular Domain)

N° du produit ABIN6937575

L’anticorps Lapin Monoclonal anti-Insulin Receptor a été validé pour IHC (f). Il convient pour détecter Insulin Receptor dans des échantillons de Humain.

Aperçu rapide pour Recombinant Insulin Receptor anticorps (Extracellular Domain) (ABIN6937575)

Antigène: Insulin Receptor (INSR)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Insulin Receptor est non-conjugé

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: INSR-2277R

Détail du produit anti-Insulin Receptor anticorps

Épitope: Extracellular Domain

Specificité: The insulin receptor (INSR) is a heterodimeric protein complex that has an intracellular subunit, which is disulfide-linked to a transmembrane segment. The insulin ligand binds to the INSR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to INSR induces phosphorylation of intra-cellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. Type 1 diabetes is an autoimmune condition of the endocrine pancreas that results in destruction of insulin secreting cells and a progressive loss in insulin-sensitive glucose uptake by cells.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment of extracellular domain of human Insulin Receptor alpha (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat cells. Human pancreas.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Insulin Receptor

Antigène: Insulin Receptor (INSR)

Autre désignation: INSR

Sujet: CD220, HHF5, HIR A, INSR, Insulin receptor, Insulin receptor subunit alpha, IR,Insulin Receptor Alpha
Cellular localisation: Cytoplasm.

Poids moléculaire: 135kDa

ID gène: 3643, 465744

UniProt: P06213

Pathways: Signalisation NF-kappaB, Signalisation RTK, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity