Laminin gamma 1 anticorps

N° du produit ABIN6937767

Détail du produit anti-Laminin gamma 1 anticorps

Antigène: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp Laminin gamma 1 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This MAb reacts with laminin B2/1 chain of ~210 kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific MAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This MAb stains uniformly all human and murine basement membranes.

Réactivité croisée (Details): Human. Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Murine EHS laminin preparation

Clone: A5

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Normal colon or carcinomas.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Laminin gamma 1

Antigène: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Autre désignation: LAMC1 (LAMC1 Produits)

Synonymes: anticorps lamb2, anticorps Lamb2, anticorps B2e, anticorps sly, anticorps LAMB2, anticorps putative laminin gamma-1 chain, anticorps laminin subunit gamma 1, anticorps laminin, gamma 1, anticorps Smp_163810, anticorps lamc1, anticorps Lamc1, anticorps LAMC1

Sujet: LAMB2, LAMC1, Laminin B2 chain, Laminin gamma 1, Laminin gamma 1 chain,Laminin, gamma 1 (LAMC1)
Cellular localisation: Basement membrane

Poids moléculaire: 210kDa

ID gène: 3915, 609663

UniProt: P11047, Q5VYE7, Q6NVY8