SUMO1 anticorps

N° du produit ABIN6938466

L’anticorps Souris Monoclonal anti-SUMO1 a été validé pour WB, IF, FACS et IHC (f). Il convient pour détecter SUMO1 dans des échantillons de Humain.

Aperçu rapide pour SUMO1 anticorps (ABIN6938466)

Antigène: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SUMO1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: SM1-495

Détail du produit anti-SUMO1 anticorps

Specificité: This MAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.

Réactivité croisée (Details): Human. Rat. Predicted to show a broad species reactivity.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human SUMO1 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Breast Carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur SUMO1

Antigène: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Autre désignation: SUMO1

Sujet: GAP-modifying protein 1, GMP1, OFC10, PIC1, SENP2, Sentrin 1, Small ubiquitin-related modifier 1, SMT3, SMT3 suppressor of mif two 3 homolog 1, SMT3C, SMT3H3, Ubiquitin homology domain protein PIC1, Ubiquitin Like 1, Ubiquitin like protein UBL1,SUMO-1
Cellular localisation: Predominantly nuclear with some cytoplasmic

Poids moléculaire: 11.5kDa (Monomer), 90kDa (Heteromer)

ID gène: 7341, 596171

UniProt: P63165

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway