ERBB4 anticorps (AA 1116-1269)

N° du produit ABIN6939332

Cet anticorps Souris Monoclonal détecte spécifiquement ERBB4 dans IF et FACS. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour ERBB4 anticorps (AA 1116-1269) (ABIN6939332)

Antigène: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ERBB4 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: HFR-1

Détail du produit anti-ERBB4 anticorps

Épitope: AA 1116-1269

Fonction: HER-4 / ERBB4 Antibody

Specificité: The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full-length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. Its expression in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in studies which suggest ErbB4 expression is involved in both normal tissue development and carcinogenesis.

Réactivité croisée (Details): Predicted to work with Rat, Chicken.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant human ERBB4 (HER4) protein fragment (around aa 1116-1269) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Information d'application

Indications d'application:

Positive Control: Breast, brain, heart or kidney. MCF-7 cells.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur ERBB4

Antigène: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Autre désignation: ERBB4

Sujet:

Avian erythroblastic leukemia viral oncogene homolog 4, ERBB4, HER4, human epidermal growth factor receptor 4, p180erbB4, Proto-oncogene-like protein c-ErbB-4

Brain, Breast, heart or kidney. MCF-7 cells.

Poids moléculaire: 180kDa (precursor), 80/120kDa (cleaved)

ID gène: 2066, 390729

UniProt: Q15303

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway