Recombinant Histone H1 anticorps

N° du produit ABIN6939601

Détail du produit anti-Histone H1 anticorps

Antigène: Histone H1

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone H1 est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Immunogène: Nuclei of human leukemia biopsy cells

Clone: AE-4

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma (IHC).

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Histone H1

Antigène: Histone H1

Autre désignation: Histone H1 (Pan Nuclear Marker) (Histone H1 Produits)

Synonymes: anticorps H10, anticorps H1FV, anticorps H1 histone family member 0, anticorps H1F0

Sujet: Please note that this antibody is a recombinant Rabbit version of original anti-histone H1 antibody (Clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a pure preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker.

Poids moléculaire: ~30kDa

ID gène: 3005