HSP27 anticorps

N° du produit ABIN6939661

Cet anticorps Souris Monoclonal détecte spécifiquement HSP27 dans WB, IHC, IF et FACS. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Singe, Mouton, Poulet et Chimpanzé.

Aperçu rapide pour HSP27 anticorps (ABIN6939661)

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivité: Humain, Souris, Rat, Singe, Mouton, Poulet, Chimpanzé

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSP27 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: G3-1

Détail du produit anti-HSP27 anticorps

Fonction: HSP27 (Heat Shock Protein 27) Antibody

Specificité: MAb G3.1 reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: MCF-7 cells. ~50 % of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR. HeLa cells.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.25-0.5 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur HSP27

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: HSPB1

Sujet:

Heat shock 27 kDa protein, Estrogen-regulated 24 kDa protein, Heat shock 25 kDa protein 1, Heat shock 28 kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)

especially those that are positive for ER and/or PgR., MCF-7 or HeLa cells. ~50 % of breast carcinomas are positive for hsp27

Poids moléculaire: 27kDa

ID gène: 3315, 520973

UniProt: P04792

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling