CD11b anticorps (AA 941-1074)

N° du produit ABIN6939810

Cet anticorps anti-CD11b est un anticorps Souris Monoclonal détectant CD11b dans IHC, WB et StM. Adapté pour Humain.

Aperçu rapide pour CD11b anticorps (AA 941-1074) (ABIN6939810)

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Staining Methods (StM)

Clone: ITGAM-3340

Détail du produit anti-CD11b anticorps

Épitope: AA 941-1074

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment (around aa941-1074) of human ITGAM protein (exact sequence is proprietary)

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: Monocytes & granulocytes. Lymph nodes, spleen and tonsil (IHC).

Known Application: Western Blot (0.5-1.0 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: ITGAM

Sujet: CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin aM combines with the Integrin '2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin aM/'2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b andCD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.

Poids moléculaire: 95kDa & 170kDa

ID gène: 3684

UniProt: P11215

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation