s100a4 anticorps (AA 1-200)

N° du produit ABIN6940541

Cet anticorps Souris Monoclonal détecte spécifiquement s100a4 dans WB, IHC, IF et FACS. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour s100a4 anticorps (AA 1-200) (ABIN6940541)

Antigène: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp s100a4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: S100A4-1481

Détail du produit anti-s100a4 anticorps

Épitope: AA 1-200

Fonction: S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody

Specificité: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, °™6 æ4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: HeLa, A549 or A375 cells (IF/FACS). HeLa, A549, T98G or A375 cell lysate (WB). Human placenta tissue (IHC).

Known Application: Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur s100a4

Antigène: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Autre désignation: S100A4

Sujet:

S100A4, S100 calcium-binding protein A4, Calvasculin, CAPL, Fibroblast specific protein 1 (FSP1), Leukemia multidrug resistance associated protein, Malignant transformation suppression 1 (MTS1), Metastasin, Placental calcium-binding protein

HeLa

Poids moléculaire: 10-12kDa

ID gène: 6275, 654444

UniProt: P26447

Pathways: S100 Proteins