Recombinant s100a4 anticorps (AA 1-200)

N° du produit ABIN6940544

Détail du produit anti-s100a4 anticorps

Antigène: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Type d'anticorp: Recombinant Antibody

Épitope: AA 1-200

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp s100a4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)

Clone: RS100A4-1481

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HeLa, A549 or A375 cells (IF/FACS). HeLa, A549, T98G or A375 cell lysate (WB). Human placenta tissue (IHC).

Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur s100a4

Antigène: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Autre désignation: S100A4 (S100A4 Produits)

Sujet: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Poids moléculaire: 10-12kDa

ID gène: 6275

UniProt: P26447

Pathways: S100 Proteins