DNMT1 anticorps (Lys142, meLys142)

N° du produit ABIN6971593

Cet anticorps anti-DNMT1 est un anticorps Lapin Polyclonal détectant DNMT1 dans WB. Adapté pour Humain.

Aperçu rapide pour DNMT1 anticorps (Lys142, meLys142) (ABIN6971593)

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNMT1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-DNMT1 anticorps

Épitope: Lys142, meLys142

Attributs du produit: DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Methylation at lysine 142 by SETD7 promotes DNMT1 proteasomal degradation while phosphorylation of serine 143 via AKT blocks degradation. These modifications appear to play an important role in regulating DNMT enzyme activity and thus levels of DNA methylation. DNMT1 monomethyl Lys142 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Human samples.

Purification: Affinity Purified

Immunogène: This antibody was raised against a peptide including monomethyl lysine 142 of human DNMT1.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur DNMT1

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Autre désignation: DNMT1

Poids moléculaire: 200 kDa

NCBI Accession: NP_001124295

Pathways: SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2