Caspase 12 anticorps (AA 170-220)

N° du produit ABIN6990234

Cet anticorps anti-Caspase 12 Polyclonal Lapin (ABIN6990234) détecte spécifiquement Caspase 12 dans WB, ELISA, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Caspase 12 anticorps (AA 170-220) (ABIN6990234)

Antigène: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Caspase 12 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Caspase 12 anticorps

Épitope: AA 170-220

Specificité: Caspase-12 (large) antibody is predicted to have no cross reactivity to other members in the caspase family.

Purification: Caspase-12 (Large) Antibody is affinity chromatography purified via peptide column.

Immunogène: Caspase-12 antibody was raised against a synthetic peptide corresponding to 16 amino acids within the large cleavage product of murine caspase-12. The immunogen is located within amino acids 170 - 220 of Caspase-12.

Isotype: IgG

Information d'application

Indications d'application: Caspase-12 (large) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 0.25 to 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Caspase-12 Antibody is supplied in PBS containing 0.02 % sodium azide. (Large)

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Caspase-12 (Large) antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur Caspase 12

Antigène: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Autre désignation: Caspase-12

Sujet: Caspase-12 Antibody: (Large)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.

Poids moléculaire: Predicted: 46 kDa

Observed: 46 kDa

ID gène: 12364

UniProt: O08736

Pathways: Apoptose, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response