ASK1 anticorps (C-Term)

N° du produit ABIN6990257

Détail du produit anti-ASK1 anticorps

Antigène: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Épitope: C-Term

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ASK1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: ASK1 Antibody is affinity chromatography purified via peptide column.

Immunogène: ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1. The sequence is different from that of mouse by last two amino acids. The immunogen is located within the last 50 amino acids of ASK1.

Isotype: IgG

Information d'application

Indications d'application: ASK1 antibody can be used for detection of ASK1 by Western blot at 0.5 μ,g/mL. A 155 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: ASK1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: ASK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur ASK1

Antigène: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Autre désignation: ASK1 (MAP3K5 Produits)

Synonymes: anticorps ASK1, anticorps MAPKKK5, anticorps MEKK5, anticorps mitogen-activated protein kinase kinase kinase 5, anticorps MUD21.11, anticorps MUD21_11, anticorps 7420452D20Rik, anticorps ASK, anticorps Mekk5, anticorps Ask1, anticorps RGD1306565, anticorps mitogen-activated protein kinase kinase kinase 5, anticorps MAP3K5, anticorps MAPKKK5, anticorps Map3k5, anticorps map3k5

Sujet: ASK1 Antibody: Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-α,-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

Poids moléculaire: 155 kDa

ID gène: 4217

UniProt: Q99683

Pathways: Signalisation MAPK, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response