AIF anticorps (AA 500-550)

N° du produit ABIN6990274

Cet anticorps Lapin Polyclonal détecte spécifiquement AIF dans WB, ELISA et IHC (p). Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour AIF anticorps (AA 500-550) (ABIN6990274)

Antigène: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AIF est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-AIF anticorps

Épitope: AA 500-550

Specificité: At least five isoforms of AIF are known to exist, this antibody will detect all isoforms except isoform 5.

Purification: AIF Antibody is affinity chromatography purified via peptide column.

Immunogène: Anti-AIF antibody was raised against a peptide corresponding to 15 amino acids near the center of human AIF. The immunogen is located within amino acids 500-550 of AIF.

Isotype: IgG

Information d'application

Indications d'application: WB: 1-2 μ,g/mL, IHC-P: 10 μ,g/mL.

Antibody validated: Western Blot in human, mouse, and rat samples, Immunohistochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: AIF Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: AIF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur AIF

Antigène: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Autre désignation: AIF

Sujet: AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.

Poids moléculaire: Predicted: 67 kDa

Observed: 68 kDa

ID gène: 9131

UniProt: O95381

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, L'effet Warburg