ELISA, Western Blotting (WB), Immunocytochemistry (ICC)
Specificité
At least two isoforms of SLC35D2 are known to exist, this antibody will recognize both isoforms. SLC35D2 antibody is predicted to not cross-react with SLC35D1 or SLC35D3.
Purification
SLC35D2 Antibody is affinity chromatography purified via peptide column.
Immunogène
SLC35D2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human SLC35D2. The immunogen is located within the last 50 amino acids of SLC35D2.
SLC35D2
Reactivité: Souris
WB, IF (cc), IF (p)
Hôte: Lapin
Polyclonal
Alexa Fluor 350
Indications d'application
SLC35D2 antibody can be used for detection of SLC35D2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SLC35D2 Antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
SLC35D2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antigène
Solute Carrier Family 35 (UDP-GlcNAc/UDP-Glucose Transporter), Member D2 (SLC35D2)
anticorps 5730408I21Rik, anticorps SQV7L, anticorps UGTrel8, anticorps hfrc, anticorps mUGTrel8, anticorps solute carrier family 35, member D2, anticorps Slc35d2
Sujet
SLC35D2 Antibody: The solute carrier family SLC35 consists of at least 17 proteins that act as nucleotide sugar transporters localized to the Golgi apparatus and endoplasmic reticulum. The role of the ER-resident SLC family member SLC35D2 is to transport both UDP-glucuronic acid and UDP-N-acetylgalactosamine. Its overexpression in transfected cells modulated cell surface heparin sulfate expression, suggesting that SLC35D2 is involved in heparin sulfate synthesis. SLC35D2-overexpressing cells also showed increased constitutive and hypotonic stress-stimulated release of UDP-GlcNAc, suggesting that SLC35D2 may be involved in UDP-sugar release and cell signaling.