DEPTOR anticorps (AA 220-270)

N° du produit ABIN6992003

Détail du produit anti-DEPTOR anticorps

Antigène: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Épitope: AA 220-270

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DEPTOR est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist, this antibody will detect all three isoforms.

Purification: DEPTOR antibody is affinity chromatography purified via peptide column.

Immunogène: DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR. The immunogen is located within amino acids 220 - 270 of DEPTOR.

Isotype: IgG

Information d'application

Indications d'application: DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: DEPTOR Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: DEPTOR antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Détails sur DEPTOR

Antigène: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Autre désignation: DEPTO (DEPTOR Produits)

Synonymes: anticorps DEP.6, anticorps DEPDC6, anticorps 4731402B04Rik, anticorps 9130412E02Rik, anticorps D15Ertd336e, anticorps D15Ertd597e, anticorps Depdc6, anticorps Depdc6-001, anticorps Depdc6-002, anticorps R75183, anticorps mKIAA4200, anticorps RGD1561030, anticorps RAPGEF4, anticorps DEP domain containing MTOR interacting protein, anticorps DEP domain containing MTOR-interacting protein, anticorps DEPTOR, anticorps Deptor

Sujet: DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).

Poids moléculaire: Predicted: 45 kDa

Observed: 42 kDa

ID gène: 64798

NCBI Accession: NP_073620

UniProt: Q8TB45

Pathways: Signalisation PI3K-Akt, Signalisation RTK, Regulation of Cell Size, BCR Signaling, L'effet Warburg