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Nestin anticorps (AA 178-399)

L’anticorps Souris Monoclonal anti-Nestin a été validé pour WB, IHC, ICC et IP. Il convient pour détecter Nestin dans des échantillons de Humain.
N° du produit ABIN7426685

Aperçu rapide pour Nestin anticorps (AA 178-399) (ABIN7426685)

Antigène

Voir toutes Nestin (NES) Anticorps
Nestin (NES)

Reactivité

  • 109
  • 40
  • 35
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Humain

Hôte

  • 69
  • 50
  • 3
  • 1
  • 1
Souris

Clonalité

  • 70
  • 54
Monoclonal

Conjugué

  • 68
  • 8
  • 5
  • 5
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Nestin est non-conjugé

Application

  • 59
  • 55
  • 37
  • 36
  • 33
  • 27
  • 20
  • 14
  • 13
  • 12
  • 8
  • 8
  • 6
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone

D10
  • Épitope

    • 16
    • 10
    • 7
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 178-399

    Fonction

    Monoclonal Antibody to Nestin (NES)

    Specificité

    The antibody is a mouse monoclonal antibody raised against NES. It has been selected for its ability to recognize NES in immunohistochemical staining and western blotting.

     Réactivité croisée

    Rat

    Purification

    Protein A + Protein G affinity chromatography

    Immunogène

    Recombinant Nestin (NES) corresdonding to Leu178~Pro399 with N-terminal His Tag

    Isotype

    IgG2b kappa
  • Indications d'application

    Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Flow cytometry: 10 μg/mL,Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

    Agent conservateur

    ProClin

    Précaution d'utilisation

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    24 months
  • Antigène

    Nestin (NES)

    Autre désignation

    Nestin

    UniProt

    P48681
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