CTNNB1 anticorps (Internal Region)

N° du produit ABIN7013874

Cet anticorps Chèvre Polyclonal détecte spécifiquement CTNNB1 dans WB, ELISA, IF et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CTNNB1 anticorps (Internal Region) (ABIN7013874)

Antigène: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CTNNB1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-CTNNB1 anticorps

Épitope: Internal Region

Fonction: CTNNB1 / catenin beta-1

Séquence: ETARCTAGTL HNLS

Specificité: Reported variants represent identical protein: NP_001091679.1, NP_001895.1, NP_001091680.1.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Information d'application

Indications d'application:

Western Blot: Approx 90 kDa band observed in A431 cell lysates-and in preliminary testing of CACO2 cell lysate (calculated MW of 85.5 kDa according to NP_001895.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of U251 and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended c

Restrictions: For Research Use only

Stockage

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur CTNNB1

Antigène: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Autre désignation: CTNNB1

Sujet: CTNNB1, catenin (cadherin-associated protein), beta 1, 88 kDa, CTNNB, MRD19, armadillo, catenin beta-1

ID gène: 1499, 12387, 84353

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2