SLC5A1 anticorps (Internal Region)

N° du produit ABIN7013885

Détail du produit anti-SLC5A1 anticorps

Antigène: SLC5A1 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1))

Épitope: Internal Region

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC5A1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Fonction: SGLT1

Séquence: KETIEIETQV PEKKK

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Information d'application

Indications d'application:

DS WB Results: Approx 75 kDa band observed in isolated Brush Border membranes from Human Kidney (calculated MW of 73.5 kDa according to NP_000334.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

DS IHC Results: In paraffin embedded Human Small Intestine shows staining of the glycocalix. Recommended concentration: 3-6 μg/mL.

Peptide ELISA Statement: Peptide ELISA: antibody detection limit dilution 1:4000.

Commentaires:

Immunocytochemistry: Fig A.Frozen sections of Human Kidney show staining of the brush borders in DCT, while only weak background was observed in Rat and Pig Kidney. Recommended concentration: 2-4ug/ml. Fig B.PFA-fixed 4μm cryosections of Human Kidney shows staining of the brush border of proximal straight tubules (S3 segments) in the outer stripe zone. The signal drops to background levels upon blocking with the immunizing peptide.

Restrictions: For Research Use only

Stockage

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin. Aliquot and store at -20 °C. Minimize freezing and thawing.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Détails sur SLC5A1

Antigène: SLC5A1 (Solute Carrier Family 5 (Sodium/glucose Cotransporter), Member 1 (SLC5A1))

Autre désignation: SLC5A1 (SLC5A1 Produits)

Synonymes: anticorps D22S675, anticorps NAGT, anticorps SGLT1, anticorps zgc:66074, anticorps zgc:85782, anticorps SLC5A1, anticorps Sglt1, anticorps solute carrier family 5 member 1, anticorps solute carrier family 5 (sodium/glucose cotransporter), member 1, anticorps SLC5A1, anticorps slc5a1, anticorps Slc5a1

Sujet: SLC5A1, solute carrier family 5 (sodium/glucose cotransporter), member 1, D22S675, NAGT, SGLT1, Human Na+/glucose cotransporter 1 mRNA, complete cds, sodium/glucose cotransporter 1, solute carrier family 5 (sodium/glucose transporter), member 1

ID gène: 6523