SLC1A5 anticorps (C-Term, Intracellular)

N° du produit ABIN7886778

Cet anticorps Lapin Polyclonal détecte spécifiquement SLC1A5 dans WB, IHC et IF. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour SLC1A5 anticorps (C-Term, Intracellular) (ABIN7886778)

Antigène: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC1A5 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-SLC1A5 anticorps

Épitope: AA 502-516, C-Term, Intracellular

Fonction: A Rabbit Polyclonal Antibody to ASCT2 Transporter

Réactivité croisée: Souris, Rat

Réactivité croisée (Details): Will not recognize ASCT2 from human samples.

Homologie: Rat - 14,15 amino acid residues identical

Attributs du produit: Anti-ASCT2 (SLC1A5) Antibody (ABIN7043668, ABIN7044823 and ABIN7044824) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry analysis. It has been designed to recognize SLC1A5 from mouse and rat samples. It will not recognize human ASCT2.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)KMPSSEPELIQVKNE, corresponding to amino acid residues 502-516 of mouse SLC1A5

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7236918)

Blocking Peptide: (ABIN7236918)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur SLC1A5

Antigène: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Autre désignation: SLC1A5

Sujet:

Synonyms: Alanine/serine/cysteine transporter 2, Neutral amino acid transporter B(0), ATB(0), ATBO, Sodium-dependent neutral amino acid transporter type 2, AAAT

Description: ASCT2 is an alanine-serine-cysteine amino acid transporter and solute linked carrier family 1 member A5 (SLC1A5). It functions as an essential high-affinity glutamine transporter. It also functions as a Na+-dependent transporter for alanine, serine and cysteine. The transport process is electroneutral which involves the Na+-coupled influx of glutamine to the Na+-coupled efflux of another substrate such as alanine, serine or cysteine. ASCT2 is expressed various peripheral tissues such as the lung and kidney1,2. ASCT2 has an important contribution to the homeostasis of amino acid metabolism within tumor cells3.ASCT2 seems to be up regulated in several cancerous tissues such as stomach, liver, kidney, breast, prostate, skin, colorectal, pancreatic, tongue and lung cancers. Studies have shown that ASCT2 is necessary for activating critical survival and cellular growth signaling cascades in cancer, which includes mTOR and ERK pathways. It has been shown that knockdown of ASCT2 in prostate cancer cell line xenograft models significantly inhibit tumor growth and metastasis in vivo. Thus, ASCT2 is a potential cancer therapeutic target1,2.

ID gène: 20514

NCBI Accession: NM_001145144

UniProt: P51912

Pathways: Dicarboxylic Acid Transport, L'effet Warburg