SLC1A5 anticorps (Extracellular)

N° du produit ABIN7043669

L’anticorps Lapin Polyclonal anti-SLC1A5 a été validé pour WB, FACS et LCI. Il convient pour détecter SLC1A5 dans des échantillons de Humain.

Aperçu rapide pour SLC1A5 anticorps (Extracellular) (ABIN7043669)

Antigène: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC1A5 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-SLC1A5 anticorps

Épitope: AA 202-217, Extracellular

Fonction: A Rabbit Polyclonal Antibody to ASCT2 Transporter

Specificité: 2nd extracellular loop

Réactivité croisée (Details): The antibody will not recognize rat or mouse ASCT2.

Homologie: Human only

Attributs du produit: Anti-Human ASCT2/SLC1A5 (extracellular) Antibody (ABIN7043669, ABIN7044825 and ABIN7044826) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize SLC1A5 from human samples only. The antibody will not recognize rat or mouse ASCT2.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSYSTTYEERNITGTR, corresponding to amino acid residues 202 - 217 of human ASCT2

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur SLC1A5

Antigène: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Autre désignation: SLC1A5

Sujet: Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2,ASCT2 is an alanine-serine-cysteine amino acid transporter and solute linked carrier family 1 member A5 (SLC1A5). It functions as an essential high-affinity glutamine transporter. It also functions as a Na+-dependent transporter for alanine, serine and cysteine. The transport process is electroneutral which involves the Na+-coupled influx of glutamine to the Na+-coupled efflux of another substrate such as alanine, serine or cysteine. ASCT2 is expressed various peripheral tissues such as the lung and kidney1,2. ASCT2 has an important contribution to the homeostasis of amino acid metabolism within tumor cells3.ASCT2 seems to be up regulated in several cancerous tissues such as stomach, liver, kidney, breast, prostate, skin, colorectal, pancreatic, tongue and lung cancers. Studies have shown that ASCT2 is necessary for activating critical survival and cellular growth signaling cascades in cancer, which includes mTOR and ERK pathways. It has been shown that knockdown of ASCT2 in prostate cancer cell line xenograft models significantly inhibit tumor growth and metastasis in vivo. Thus, ASCT2 is a potential cancer therapeutic target1,2.

Alternative names: Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2, ASCT2, SLC1A5

ID gène: 6510

NCBI Accession: NM_001145144

UniProt: Q15758

Pathways: Dicarboxylic Acid Transport, L'effet Warburg