SLC1A5 anticorps (2nd Extracellular Loop)

N° du produit ABIN7886779

L’anticorps anti-SLC1A5 Polyclonal Lapin est utilisé pour la détection de SLC1A5 dans des échantillons de Humain. Il a été validé pour WB, FACS et LCI.

Aperçu rapide pour SLC1A5 anticorps (2nd Extracellular Loop) (ABIN7886779)

Antigène: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC1A5 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-SLC1A5 anticorps

Épitope: 2nd Extracellular Loop, AA 202-217

Fonction: A Rabbit Polyclonal Antibody to ASCT2 Transporter

Réactivité croisée (Details): The antibody will not recognize rat or mouse ASCT2.

Homologie: Human only

Attributs du produit: Anti-Human ASCT2/SLC1A5 (extracellular) Antibody (ABIN7043669, ABIN7044825 and ABIN7044826) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize SLC1A5 from human samples only. The antibody will not recognize rat or mouse ASCT2.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)RSYSTTYEERNITGTR, corresponding to amino acid residues 202 - 217 of human ASCT2

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur SLC1A5

Antigène: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Autre désignation: SLC1A5

Sujet:

Synonyms: Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2

Description: ASCT2 is an alanine-serine-cysteine amino acid transporter and solute linked carrier family 1 member A5 (SLC1A5). It functions as an essential high-affinity glutamine transporter. It also functions as a Na+-dependent transporter for alanine, serine and cysteine. The transport process is electroneutral which involves the Na+-coupled influx of glutamine to the Na+-coupled efflux of another substrate such as alanine, serine or cysteine. ASCT2 is expressed various peripheral tissues such as the lung and kidney1,2. ASCT2 has an important contribution to the homeostasis of amino acid metabolism within tumor cells3.ASCT2 seems to be up regulated in several cancerous tissues such as stomach, liver, kidney, breast, prostate, skin, colorectal, pancreatic, tongue and lung cancers. Studies have shown that ASCT2 is necessary for activating critical survival and cellular growth signaling cascades in cancer, which includes mTOR and ERK pathways. It has been shown that knockdown of ASCT2 in prostate cancer cell line xenograft models significantly inhibit tumor growth and metastasis in vivo. Thus, ASCT2 is a potential cancer therapeutic target1,2.

ID gène: 6510

NCBI Accession: NM_001145144

UniProt: Q15758

Pathways: Dicarboxylic Acid Transport, L'effet Warburg