Recombinant RANKL anticorps

N° du produit ABIN7072529

L’anticorps Lapin Chimeric anti-RANKL a été validé pour IHC, FACS et Neut. Il convient pour détecter RANKL dans des échantillons de Souris.

Aperçu rapide pour Recombinant RANKL anticorps (ABIN7072529)

Antigène: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp RANKL est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Neutralization (Neut)

Clone: IK22-5

Détail du produit anti-RANKL anticorps

Fonction: Anti-RANKL [IK22-5], Rabbit IgG, kappa

Specificité: This antibody is specific for murine RANKL.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: Thus antibody was raised by immunising an SD rat with murine RANKL-transfected baby hamster kidney cells.

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody has been used in immunohistochemical analysis of frozen Peyer's patch tissue sections (Kobayashi et al, 2013). It has also been used in flow cytometric analysis of RANKL cell surface expression by Th1 and Th17 cells (Nakae et al, 2007), RANKL-transfected human embryonic kidney cells (Miyahira et al, 2003) and murine T hybridoma 2B4.11 cells (Fionda et al, 2007). Additionally, this RANKL-neutralising antibody has been used to assess the effects of RANKL inhibition on myoepithelial cell proliferation in mammary organoid culture (Haslam et al, 2008). Treatment of collagen-induced arthritis (CIA) mice with this antibody has been shown to reduce bone loss at the site of inflammation (Kamijo et al, 2006), while administration to C57BL/6J mice induced transient tibia and skull bone growth arrest and irreversible blockage of tooth eruption (Lezot et al, 2015).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur RANKL

Antigène: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Autre désignation: RANKL

Sujet: CD254, IK22/5, RANK Ligand, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, TRANCE

UniProt: O35235

Pathways: Signalisation NF-kappaB