CHEK2 anticorps

N° du produit ABIN7126216

Cet anticorps anti-CHEK2 est un anticorps Souris Monoclonal détectant CHEK2 dans WB, IF, IP et FACS. Adapté pour Humain.

Aperçu rapide pour CHEK2 anticorps (ABIN7126216)

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CHEK2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Détail du produit anti-CHEK2 anticorps

Specificité: Recognizes a serine/threonine protein kinase that is a required check-point to mediate cell cycle arrest, activation of DNA repair and apoptosis. In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. Also, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.

Réactivité croisée (Details): Human. Predicted to react with Mouse and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant fragment of human CHEK2 corresponding to protein domain

Isotype: IgG2b

Information d'application

Indications d'application:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), ,Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HCT 116 cells. Human testis, spleen or colon.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur CHEK2

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Autre désignation: CHEK2

Sujet: Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, CHK2 checkpoint homolog (S. pombe), hCds1, HuCds 1, LFS2, PP1425, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2,CHEK2
Cellular localisation: Nucleus.

Poids moléculaire: 60.91kDa

ID gène: 11200, 291363, 505297

UniProt: O96017

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire