EIF2C3 anticorps

N° du produit ABIN7126312

Détail du produit anti-EIF2C3 anticorps

Antigène: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EIF2C3 est non-conjugé

Application: ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS)

Specificité: This antigen is involved in binding and translation repression of short RNAs. This antigen is also known as argonaute RISC catalytic component 3.Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.

Réactivité croisée (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant full-length human AGO3 protein

Isotype: IgG1

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, MCF7 or U-2OS cells.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur EIF2C3

Antigène: EIF2C3 (Eukaryotic Translation Initiation Factor 2C3 (EIF2C3))

Autre désignation: AGO3 (EIF2C3 Produits)

Synonymes: anticorps EIF2C3, anticorps Eif2c3, anticorps Argonaute3, anticorps ago3, anticorps eif2c3, anticorps eif2c3b, anticorps si:dkey-3n22.3, anticorps AW048688, anticorps C130014L07Rik, anticorps ARGONAUTE 3, anticorps T19E23.8, anticorps T19E23_8, anticorps argonaute 3, RISC catalytic component, anticorps argonaute RISC catalytic component 3b, anticorps argonaute RISC catalytic subunit 3, anticorps argonaute RISC catalytic component 3, anticorps ARGONAUTE 3, anticorps AGO3, anticorps Ago3, anticorps ago3b

Sujet: Argonaute 3, Argonaute3, eIF 2C 3, eIF-2C 3, eIF2C 3, Eif2c3, EIF2C3 protein, Eukaryotic translation initiation factor 2C 3, Eukaryotic translation initiation factor 2C3, FLJ12765, hAgo3, MGC86946, Protein argonaute-3,AGO3 (Argonaute-3) / eIF2C3
Cellular localisation: Cytoplasm>P-bodies. Nucleoplasm.

Poids moléculaire: 97.36kDa

ID gène: 192669, 657659

UniProt: Q9H9G7

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway