CHEK2 anticorps (pThr383)

N° du produit ABIN7138897

Cet anticorps anti-CHEK2 est un anticorps Lapin Polyclonal détectant CHEK2 dans WB, ELISA et IF. Adapté pour Humain.

Aperçu rapide pour CHEK2 anticorps (pThr383) (ABIN7138897)

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Détail du produit anti-CHEK2 anticorps

Épitope: pThr383

Fonction: Phospho-CHEK2 (Thr383) Antibody

Réactivité croisée: Souris, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogène: Peptide sequence around phosphorylation site of threonine383 (M-R-T(p)-L-C) derived from Human Chk1.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur CHEK2

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Autre désignation: CHEK2

Sujet:

Background:

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by Chk2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

Shannon L. Gibson, Cancer Res., Dec 2005, 65: 10734 - 10741.
Jinwoo Ahn, J. Biol. Chem., Dec 2002, 277: 48418 - 48426.
Nabil H. Chehab, Genes & Dev., Feb 2000, 14: 278.

Aliases: CDS 1 antibody, Cds1 antibody, Cds1 homolog antibody, Checkpoint kinase 2 antibody, Checkpoint like protein CHK2 antibody, CHEK 2 antibody, Chek2 antibody, Chk 2 antibody, CHK2 checkpoint homolog (S. pombe) antibody, CHK2 checkpoint homolog antibody, CHK2_HUMAN antibody, hCds1 antibody, HuCds 1 antibody, LFS 2 antibody, LFS2 antibody, PP1425 antibody, RAD 53 antibody, RAD53 antibody, Rad53 homolog antibody, Serine/threonine protein kinase Chk2 antibody, Serine/threonine-protein kinase Chk2 antibody

UniProt: O96017

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire