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HIST1H3A anticorps (acLys27)

Name: HIST1H3A anticorps (acLys27)
Brand: antibodies-online
SKU: ABIN7139175
Price: 380.9 EUR
Availability: InStock

HIST1H3A Reactivité: Humain ELISA, WB, IF, ChIP, ICC, IP Hôte: Lapin Polyclonal unconjugated

Western Blot Detected samples: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, Untreated (-) or treated (+) with 30 mM sodium butyrate for 4h All lanes: HIST1H3A antibody at 1:100 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa (HIST1H3A anticorps (acLys27))

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with ABIN7139175 at 1:5, counter-stained with DAPI. (HIST1H3A anticorps (acLys27))

Immunocytochemistry analysis of ABIN7139175 diluted at 1:10 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. (HIST1H3A anticorps (acLys27))

ICC

Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30 mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5 μg anti-HIST1H3A (ABIN7139175) or a control normal rabbit IgG. (HIST1H3A anticorps (acLys27))

IHC

Immunoprecipitating HIST1H3A in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of ABIN7139175 in HepG2 whole cell lysate. (HIST1H3A anticorps (acLys27))

5 images

N° du produit ABIN7139175

Fiche de données

Antigène Voir toutes HIST1H3A Anticorps

HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))
Épitope
16

8

6

6

6

6

5

5

5

5

5

5

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
acLys27
Reactivité
242

67

50

16

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1
Humain
Hôte
235

6

1
Lapin
Anticorps secondaires appropriés
Clonalité
176

66
Polyclonal
Conjugué
223

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp HIST1H3A est non-conjugé
Application
202

182

72

68

61

57

52

27

19

17

16

13

13

7

5
ELISA, Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Réactivité croisée

Humain

Purification

Antigen Affinity Purified

Immunogène

Peptide sequence around site of Acetyl-Lys (27) derived from Human Histone H3.1

Isotype

IgG

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anti-Histone Cluster 1, H3a (HIST1H3A) (3meLys27), (pSer28) antibody
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anti-Histone Cluster 1, H3a (HIST1H3A) (acLys4) antibody
HIST1H3A Reactivité: Humain ELISA, WB, IF, ChIP, DB, ChIP-seq Hôte: Lapin Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (acLys18) antibody
HIST1H3A Reactivité: Humain, Souris ELISA, WB, IF, ChIP, DB, ChIP-seq Hôte: Lapin Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) antibody
HIST1H3A Reactivité: Humain WB, IHC, IF, ChIP Hôte: Lapin Monoclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (2meArg17 (asymetric)), (acLys18) antibody
HIST1H3A Reactivité: Humain, Souris ELISA, WB, IF, ChIP, DB, ChIP-seq Hôte: Lapin Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (meLys4) antibody
HIST1H3A Reactivité: Humain, Souris ELISA, WB, IF, ChIP, DB, ChIP-seq Hôte: Lapin Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (3meLys9) antibody
HIST1H3A Reactivité: Humain, Souris ELISA, WB, IF, ChIP, DB, ChIP-seq Hôte: Lapin Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (3meLys36) antibody
HIST1H3A Reactivité: Humain, Souris, Arabidopsis, Oryza sativa ELISA, WB, IF, ChIP, DB, ChIP-seq Hôte: Lapin Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (acLys14), (acLys9) antibody
HIST1H3A Reactivité: Humain, Souris, Poisson zèbre (Danio rerio), Arabidopsis, Truite arc-en-ciel, Aspergillus nidulans ELISA, WB, IF, ChIP, DB, ChIP-seq Hôte: Lapin Polyclonal unconjugated

Indications d'application

Recommended dilution: WB:1:100-1:1000, ICC:1:10-1:100, IF:1:1-1:10, IP:1:200-1:2000,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Agent conservateur

ProClin

Précaution d'utilisation

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

-20 °C,-80 °C

Stockage commentaire

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Antigène

HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Autre désignation

HIST1H3A (HIST1H3A Produits)

Synonymes

anticorps H3/A, anticorps H3FA, anticorps histone cluster 1 H3 family member a, anticorps histone cluster 1, H3a, anticorps HIST1H3A, anticorps Hist1h3a

Sujet

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Aliases: H3 histone family member E pseudogene antibody, H3 histone family, member A antibody, H3/A antibody, H31_HUMAN antibody, H3F3 antibody, H3FA antibody, Hist1h3a antibody, HIST1H3B antibody, HIST1H3C antibody, HIST1H3D antibody, HIST1H3E antibody, HIST1H3F antibody, HIST1H3G antibody, HIST1H3H antibody, HIST1H3I antibody, HIST1H3J antibody, HIST3H3 antibody, histone 1, H3a antibody, Histone cluster 1, H3a antibody, Histone H3 3 pseudogene antibody, Histone H3.1 antibody, Histone H3/a antibody, Histone H3/b antibody, Histone H3/c antibody, Histone H3/d antibody, Histone H3/f antibody, Histone H3/h antibody, Histone H3/i antibody, Histone H3/j antibody, Histone H3/k antibody, Histone H3/l antibody

UniProt

P68431