CHEK2 anticorps (Thr68)

N° du produit ABIN7179991

Cet anticorps anti-CHEK2 est un anticorps Lapin Polyclonal détectant CHEK2 dans WB, ELISA et IHC. Adapté pour Humain.

Aperçu rapide pour CHEK2 anticorps (Thr68) (ABIN7179991)

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-CHEK2 anticorps

Épitope: Thr68

Fonction: CHEK2 (Ab-68) Antibody

Réactivité croisée: Souris, Rat

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized non-phosphopeptide derived from Human Chk2 around the phosphorylation site of threonine 68.

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,-80 °C

Stockage commentaire: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Détails sur CHEK2

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Autre désignation: CHEK2

Sujet:

Background: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.

Bhoumik A, et al. (2005) Mol Cell, 18(5): 577-87.
Gorgoulis VG, et al. (2005) Nature, 434(7035): 907-13.
Falck J, et al. (2005) Nature, 434(7033): 605-11.
Jin ZH, et al. (2005) Oncogene, 24(12): 1973-81.
Li J, et al. (2005) J Biol Chem, 280(12): 12041-50.

Aliases: CDS 1 antibody, Cds1 antibody, Cds1 homolog antibody, Checkpoint kinase 2 antibody, Checkpoint like protein CHK2 antibody, CHEK 2 antibody, Chek2 antibody, Chk 2 antibody, CHK2 checkpoint homolog (S. pombe) antibody, CHK2 checkpoint homolog antibody, CHK2_HUMAN antibody, hCds1 antibody, HuCds 1 antibody, LFS 2 antibody, LFS2 antibody, PP1425 antibody, RAD 53 antibody, RAD53 antibody, Rad53 homolog antibody, Serine/threonine protein kinase Chk2 antibody, Serine/threonine-protein kinase Chk2 antibody

UniProt: O96017

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire