CHEK2 anticorps (pSer516)

N° du produit ABIN7212918

Cet anticorps anti-CHEK2 est un anticorps Lapin Polyclonal détectant CHEK2 dans WB et ELISA. Adapté pour Humain et Singe.

Aperçu rapide pour CHEK2 anticorps (pSer516) (ABIN7212918)

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivité: Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHEK2 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-CHEK2 anticorps

Épitope: pSer516

Fonction: Chk2 (phospho Ser516) Polyclonal Antibody

Specificité: Phospho-Chk2 (S516) Polyclonal Antibody detects endogenous levels of Chk2 protein only when phosphorylated at S516.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogène: Synthesized peptide derived from human Chk2 Phospho-Ser516

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Commentaires:

Primary Antibody

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Détails sur CHEK2

Antigène: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Autre désignation: Chk2

Sujet: Rabbit Anti-Chk2 (phospho Ser516) Polyclonal Antibody,CHEK2, CDS1, CHK2, RAD53, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Hucds1, hCds1, Checkpoint kinase 2,In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. Checkpoint kinase 2 encoded by CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, Checkpoint kinase 2 interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for CHEK2.,serine threonine-protein kinase Chk2

Poids moléculaire: observerd band 61kDa

ID gène: 11200

UniProt: O96017

Pathways: Signalisation p53, Apoptose, Cycle Cellulaire