MDM4-binding Protein anticorps (pSer367)

N° du produit ABIN7222412

Cet anticorps Lapin Polyclonal détecte spécifiquement MDM4-binding Protein dans WB, ELISA, IHC et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour MDM4-binding Protein anticorps (pSer367) (ABIN7222412)

Antigène: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MDM4-binding Protein est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-MDM4-binding Protein anticorps

Épitope: pSer367

Fonction: Rabbit Anti-MDMX (phospho Ser367) Polyclonal Antibody

Specificité: Phospho-MDMX (S367) Polyclonal Antibody detects endogenous levels of MDMX protein only when phosphorylated at S367.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human MDMX Phospho-Ser367

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur MDM4-binding Protein

Antigène: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Autre désignation: MDMX

Sujet: MDM4, MDMX, Protein Mdm4, Double minute 4 protein, Mdm2-like p53-binding protein, Protein Mdmx, p53-binding protein Mdm4MDM4 encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Poids moléculaire: 80kD

ID gène: 4194

UniProt: O15151

Pathways: Cycle Cellulaire