PKC theta anticorps

N° du produit ABIN7223558

L’anticorps anti-PKC theta Polyclonal Lapin est utilisé pour la détection de PKC theta dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB, IHC, ELISA et IF.

Aperçu rapide pour PKC theta anticorps (ABIN7223558)

Antigène: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC theta est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-PKC theta anticorps

Fonction: Rabbit Anti-PKC θ Polyclonal Antibody

Specificité: PKC θ Polyclonal Antibody detects endogenous levels of PKC θ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human PKC theta around the non-phosphorylation site of T538

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur PKC theta

Antigène: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Autre désignation: PKC theta

Sujet: PRKCQ, PRKCT, Protein kinase C theta type, nPKC-thetaProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by PRKCQ (protein kinase C theta) is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.

Poids moléculaire: 78kD

ID gène: 5588

UniProt: Q04759

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling