Crk anticorps (pTyr221)

N° du produit ABIN7213196

L’anticorps anti-Crk Polyclonal Lapin est utilisé pour la détection de Crk dans des échantillons de Humain, Souris, Rat et Singe. Il a été validé pour WB, ELISA et IF.

Aperçu rapide pour Crk anticorps (pTyr221) (ABIN7213196)

Antigène: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Crk est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Détail du produit anti-Crk anticorps

Épitope: pTyr221

Fonction: Crk II (phospho Tyr221) Polyclonal Antibody

Specificité: Phospho-Crk II (Y221) Polyclonal Antibody detects endogenous levels of Crk II protein only when phosphorylated at Y221.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogène: Synthesized peptide derived from human Crk II Phospho-Tyr221

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Commentaires:

Primary Antibody

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Détails sur Crk

Antigène: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Autre désignation: Crk II

Sujet: Rabbit Anti-Crk II (phospho Tyr221) Polyclonal Antibody,CRK, Adapter molecule crk, Proto-oncogene c-Crk, p38,CRK encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of CRK (CRK proto-oncogene, adaptor protein) has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.,Adapter molecule crk

Poids moléculaire: observerd band 40kDa

ID gène: 1398

UniProt: P46108

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor