SMAD4 anticorps (C-Term)

N° du produit ABIN7464820

L’anticorps Lapin Polyclonal anti-SMAD4 a été validé pour WB, IF, ICC et IHC (p). Il convient pour détecter SMAD4 dans des échantillons de Humain.

Aperçu rapide pour SMAD4 anticorps (C-Term) (ABIN7464820)

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SMAD4 anticorps

Épitope: C-Term

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the C-terminus region of human SMAD4. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2 , DDDDK-tagged SMAD4-transfected 293T

Validation: Comparison, Overexpression

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.58 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur SMAD4

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Autre désignation: SMAD family member 4

Sujet: SMAD family member 4 , DPC4 , JIP , MADH4 , MYHRS,This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq]

Poids moléculaire: 60 kDa

ID gène: 4089

UniProt: Q13485

Pathways: Cycle Cellulaire, Chromatin Binding, Autophagy