SQSTM1 anticorps (Internal Region)

N° du produit ABIN7540593

N° du produit (Fournisseur): 600-401-hb8

L’anticorps Lapin Polyclonal anti-SQSTM1 a été validé pour WB, IHC, IF, ELISA et FM. Il convient pour détecter SQSTM1 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour SQSTM1 anticorps (Internal Region) (ABIN7540593)

Antigène: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SQSTM1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Fluorescence Microscopy (FM)

Détail du produit anti-SQSTM1 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-hb8

Fournisseur: Rockland

Fonction: SQSTM1/p62 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human SQSTM1/p62.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène: Anti-SQSTM1/p62 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human SQSTM1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:100

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 1:1000

Commentaires:

Anti-SQSTM1/p62 Antibody has been tested in ELISA, WB, IF, and IHC.  Expect a band at ~38, 47kDa in western blot using appropriate tissues and lysates.  Positive control used: U87-MG, HeLa, and Mouse liver lysates in western blot, Human skeletal muscle in Immunohistochemistry, and HeLa in immunofluorescence.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-SQSTM1 (ABIN7540593)

Dalle Carbonare, Minoia, Vareschi, Piritore, Zouari, Gandini, Meneghel, Elia, Lorenzi, Antoniazzi, Pessoa, Zipeto, Romanelli, Guardavaccaro, Valenti: "Exploring the Interplay of RUNX2 and CXCR4 in Melanoma Progression." dans: Cells, Vol. 13, Issue 5, (2024) (PubMed).

Détails sur SQSTM1

Antigène: SQSTM1 (Sequestosome 1 (SQSTM1))

Autre désignation: SQSTM1

Sujet: Rabbit Anti-Sequestosome 1 Antibody, Phosphotyrosine-Independent Ligand For The Lck SH2 Domain Of 62 KDa, EBI3-Associated Protein Of 60 KDa, Ubiquitin-Binding Protein P62, Oxidative Stress Induced Like, EBIAP, OSIL, P60, Phosphotyrosine Independent Ligand For The Lck SH2 Domain P62, EBI3-Associated Protein P60, Paget Disease Of Bone, Sequestosome-1, FTDALS3, NADGP, A170, DMRV, ZIP3, P62B, PDB3, ORCA, P62,SQSTM1(Sequestosome 1)/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It functions as a bridge between polyubiquitinated cargo and autophagosomes and interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family.  Along with WDFY3, SQSTM1 is involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures) and is required to recruit ubiquitinated proteins to PML bodies in the nucleus. It may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1 and may play a role in titin/TTN downstream signaling in muscle cells. It is an adapter that mediates the interaction between TRAF6 and CYLD.  SQSTM1 may be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. It is involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Mutations in this gene result in frontotemporal dementia and/or amyotrophic lateral sclerosis, and familial Paget disease of bone.  Anti-SQSTM1 Antibody is useful for researchers interested in Cancer Research, Neuroscience, and Cytokines & Growth Factor Antibodies. 

ID gène: 8878

NCBI Accession: NP_001135770

UniProt: Q13501

Pathways: Signalisation NF-kappaB, Neurotrophin Signaling Pathway, Autophagy