HTR2A anticorps (Extracellular)

N° du produit ABIN7581898

Cet anticorps Cobaye Polyclonal détecte spécifiquement HTR2A dans WB et IHC. Il présente une réactivité envers Rat.

Aperçu rapide pour HTR2A anticorps (Extracellular) (ABIN7581898)

Antigène: HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))

Reactivité: Rat

Hôte: Cobaye

Clonalité: Polyclonal

Conjugué: Cet anticorp HTR2A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-HTR2A anticorps

Épitope: AA 40-52, Extracellular

Fonction: A Guinea Pig Polyclonal Antibody to 5-HT2A Receptor

Séquence: (C)SEASNWTIDA ENR

Specificité: Extracellular, N-terminus

Homologie: Mouse - identical, human - 9 out of 13 amino acid residues identical

Attributs du produit: Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (ABIN7581898) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5-HT2A from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-5HT2A Receptor (HTR2A) (extracellular) (ABIN7042875, ABIN7045286 and ABIN7045287) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation and more.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)SEASNWTIDAENR, corresponding to amino acid residues 40-52 of rat 5-Hydroxytryptamine receptor 2A

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7234591)

Blocking Peptide: (ABIN7234591)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur HTR2A

Antigène: HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))

Autre désignation: HTR2A

Sujet: 5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A, 5-HT2A,5-HT is involved in a diverse array of physiological and biological processes. In the brain, 5-HT affects sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A, 5-HT2B, and 5-HT2C2. They are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.5-HT2A receptors have been found in many regions of the human brain including the cortex, brainstem, olfactory bulb, limbic system and basal ganglia3.The 5-HT2A receptor has been implicated in many mental disorders including schizophrenia, depression, obsessive compulsive disorder (OCD), attention deficit-hyperactivity disorder (ADHD), and eating disorders such as anorexia nervosa and autism spectrum disorders4.

ID gène: 29595

UniProt: P14842

Pathways: Signalistation JAK/STAT, Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process