VPS4A anticorps (AA 1-71)

N° du produit ABIN7599531

Cet anticorps anti-VPS4A est un anticorps Lapin Polyclonal détectant VPS4A dans WB, IHC, ELISA et FACS. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour VPS4A anticorps (AA 1-71) (ABIN7599531)

Antigène: VPS4A (Vacuolar Protein Sorting-Associated Protein 4A (VPS4A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VPS4A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Détail du produit anti-VPS4A anticorps

Épitope: AA 1-71

Fonction: Anti-VPS4A Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-VPS4A Antibody Picoband® (ABIN7599531). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human VPS4A recombinant protein (Position: M1-K71).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Scheuring, S., Rohricht, R. A., Schoning-Burkhardt, B., Beyer, A., Muller, S., Abts, H. F., Kohrer, K. Mammalian cells express two VPS4 proteins both of which are involved in intracellular protein trafficking. J. Molec. Biol. 312: 469-480, 2001. 2. Scott, A., Gaspar, J., Stuchell-Brereton, M. D., Alam, S. L., Skalicky, J. J., Sundquist, W. I. Structure and ESCRT-III protein interactions of the MIT domain of human VPS4A. Proc. Nat. Acad. Sci. 102: 13813-13818, 2005. 3. Stuchell-Brereton, M. D., Skalicky, J. J., Kieffer, C., Karren, M. A., Ghaffarian, S., Sundquist, W. I. ESCRT-III recognition by VPS4 ATPases. Nature 449: 740-744, 2007.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur VPS4A

Antigène: VPS4A (Vacuolar Protein Sorting-Associated Protein 4A (VPS4A))

Autre désignation: VPS4A

Sujet:

Synonyms: Vacuolar protein sorting-associated protein 4A, Protein SKD2, VPS4-1, hVPS4, VPS4A, VPS4

Tissue Specificity: Ubiquitously expressed.

Background: Vacuolar protein sorting-associated protein 4A is a protein that in humans is encoded by the VPS4A gene. It is mapped to 16q22.1. The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16, the gene for the other resides on chromosome 18.

Poids moléculaire: 50 kDa

ID gène: 27183

Pathways: Dynamique des Microtubules, CXCR4-mediated Signaling Events