MFGE8 anticorps (AA 23-463)

N° du produit ABIN7600756

L’anticorps Lapin Polyclonal anti-MFGE8 a été validé pour WB, IHC, ELISA et FACS. Il convient pour détecter MFGE8 dans des échantillons de Souris et Rat.

Aperçu rapide pour MFGE8 anticorps (AA 23-463) (ABIN7600756)

Antigène: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MFGE8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Détail du produit anti-MFGE8 anticorps

Épitope: AA 23-463

Fonction: Anti-Milk Fat Globule 1/Mfge8 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Milk Fat Globule 1/Mfge8 Antibody Picoband® (ABIN7600756). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived mouse Milk Fat Globule 1/Mfge8 recombinant protein (Position: A23-C463). Mouse?Mfge8 shares?86.2%?amino?acid?(aa)?sequence?identity?with rat Mfge8.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Bu, H.-F., Zuo, X.-L., Wang, X., Ensslin, M. A., Koti, V., Hsueh, W., Raymond, A. S., Shur, B. D., Tan, X.-D. Milk fat globule-EGF factor 8/lactadherin plays a crucial role in maintenance and repair of murine intestinal epithelium. J. Clin. Invest. 117: 3673-3683, 2007. 2. Collins, C., Nehlin, J. O., Stubbs, J. D., Kowbel, D., Kuo, W.-L., Parry, G. Mapping of a newly discovered human gene homologous to the apoptosis associated-murine mammary protein, MFG-E8, to chromosome 15q25. Genomics 39: 117-118, 1997. 3. Hanayama, R., Tanaka, M., Miwa, K., Shinohara, A., Iwamatsu, A., Nagata, S. Identification of a factor that links apoptotic cells to phagocytes. Nature 417: 182-187, 2002.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur MFGE8

Antigène: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Autre désignation: Mfge8

Sujet:

Synonyms: Tumor necrosis factor receptor superfamily member 4, MRC OX40, OX40 antigen, OX40L receptor, CD134, Tnfrsf4, Ox40, Txgp1l

Background: MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al. (1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al. (2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al. (2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.

Poids moléculaire: 65-70 kDa

ID gène: 17304

UniProt: P21956

Pathways: SARS-CoV-2 Protein Interactome