Nestin anticorps (AA 698-844) (CF®405S)

N° du produit ABIN7657808

L’anticorps Souris Monoclonal anti-Nestin a été validé pour IHC et IHC (fp). Il convient pour détecter Nestin dans des échantillons de Humain.

Aperçu rapide pour Nestin anticorps (AA 698-844) (CF®405S) (ABIN7657808)

Antigène: Nestin (NES)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nestin est conjugé à/à la CF®405S

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: NES-2911

Détail du produit anti-Nestin anticorps (CF®405S)

Épitope: AA 698-844

Fonction: Nestin (Cancer Stem Cell Marker)(NES/2911), CF405S conjugate

Attributs du produit: Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or -internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differentiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human NES protein fragment around aa 698-844

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Positive Control: Human kidney, ovary, cervix, breast, colon, endometrium, cerebellum, lung, prostate, skin and human melanoma tissue. HeLa cell lysate. SH-SY5Y cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Stock: 4 °C

Stockage commentaire:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Date de péremption: 24 months

Détails sur Nestin

Antigène: Nestin (NES)

Autre désignation: Nestin

Sujet:

Synonyms: ESTM 46, FLJ21841, Intermediate filament protein, Nbla00170, nes

Gene Symbol: NES

Poids moléculaire: 190-200 kDa

ID gène: 10763

UniProt: P48681