KLRC1 anticorps

N° du produit ABIN7835809

L’anticorps anti-KLRC1 Monoclonal Rat est utilisé pour la détection de KLRC1 dans des échantillons de Souris. Il a été validé pour FACS et IF.

Aperçu rapide pour KLRC1 anticorps (ABIN7835809)

Antigène: KLRC1 (Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp KLRC1 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 20D5

Détail du produit anti-KLRC1 anticorps

Fonction: Rat monoclonal antibody to CD159a/c/e

Specificité: Recognizes mouse CD159a/c/e

Purification: The antibody was purified by affinity chromatography.

Immunogène: C57BL/6 mouse CD94/NKG2A transfected CHO cells

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur KLRC1

Antigène: KLRC1 (Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1))

Autre désignation: KLRC1

Sujet: NKG2A, NKG2B, CD159a, Klrc1 killer cell lectin-like receptor subfamily C member 1

ID gène: 16641