CD8B anticorps

N° du produit ABIN7836671

Cet anticorps anti-CD8B Monoclonal Rat (Clone 53-5-8) (ABIN7836671) détecte spécifiquement CD8B dans FACS et IF. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour CD8B anticorps (ABIN7836671)

Antigène: CD8B (CD8b Molecule (CD8B))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8B est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 53-5-8

Détail du produit anti-CD8B anticorps

Fonction: Rat monoclonal antibody to CD8b.2

Specificité: Recognizes mouse CD8b.2

Purification: The antibody was purified by affinity chromatography.

Immunogène: Mouse thymus or spleen

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur CD8B

Antigène: CD8B (CD8b Molecule (CD8B))

Autre désignation: CD8B

Sujet: CD8B1, T-cell surface glycoprotein CD8 beta chain, CD8b

ID gène: 12526

Pathways: TCR Signaling