BRAF anticorps

N° du produit ABIN7863851

Cet anticorps anti-BRAF Monoclonal Souris (Clone BRAF-1626) (ABIN7863851) détecte spécifiquement BRAF dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour BRAF anticorps (ABIN7863851)

Antigène: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BRAF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: BRAF-1626

Détail du produit anti-BRAF anticorps

Fonction: BRAF Antibody

Specificité: The 79oΩBRAF 79oΩgene encodes a cytoplasmic serine-threonine kinase, which initiates the activation of the mitogen-activated protein kinase (MAPK) signalling pathway. The oncogenic mutations in the kinase region of BRAF gene result in constitutive activation of the MAPK signalling pathway, leading to increased cell proliferation, resistance to apoptosis and tumor progression. The most common of all activating BRAF mutations leads to a substitution of valine (V) to glutamic acid (E) at the position 600 of the amino acid sequence. The 79oΩBRAF 79oΩV600E mutation is an important predictive and prognostic biomarker. The BRAF V600E mutation is detected in approximately 8 % of all solid tumours, including 45 % of papillary thyroid carcinomas, 40-60 % of melanomas, 5-15 % of colorectal adenocarcinomas, 35 % of serous low grade and borderline ovarian carcinomas, 1-3 % of non-small cell lung cancers, and 5-7 % of cholangiocarcinomas. 79oΩFurthermore, the BRAF V600E mutation is found in 100 % of hairy cell leukaemia, 79oΩ54 % Erdheim-Chester disease, 38 % of Langerhans cell histocytoses 79oΩand 60 % of pleomorphic xanthoastrocytomas. 79oΩ

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment of human HMG20B protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Détails sur BRAF

Antigène: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Autre désignation: BRAF

Sujet:

BRAF25, BRAF35, BRCA2-associated factor 35, High Mobility Group Protein 20B (HM20B), HMG box-containing protein 20B, HMG domain-containing protein HMGX2, HMG20B, SMARCE1-related protein, SMARCE1r, Sox-like transcriptional factor, SOXL

A431, Jurkat and HeLa cells. Melanoma or Colon Carcinoma, Human Lung, Small Intestine, Gallbladder, Pancreas tissue

Poids moléculaire: 35kDa (predicted)

ID gène: 673, 406534

UniProt: Q9P0W2

Pathways: Signalisation MAPK, Signalisation RTK, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy