IRAK1 anticorps (pSer376)

N° du produit ABIN7874123

Cet anticorps Lapin Polyclonal détecte spécifiquement IRAK1 dans WB. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour IRAK1 anticorps (pSer376) (ABIN7874123)

Antigène: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IRAK1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-IRAK1 anticorps

Épitope: AA 348-381, pSer376

Fonction: Phospho-IRAK1 Antibody (S376)

Purification: Antigen affinity

Immunogène: A portion of amino acids 348-381 from human IRAK1 was used as the immunogen for the phospho-IRAK1 antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the phospho-IRAK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the phospho-IRAK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur IRAK1

Antigène: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Autre désignation: IRAK1

Sujet: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor- signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3. [UniProt]

UniProt: P51617

Pathways: Signalisation NF-kappaB, Signalisation TLR, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades